Journal: Journal of Biological Chemistry

Article Title: Expression Profiles of Arabidopsis thaliana in Mineral Deficiencies Reveal Novel Transporters Involved in Metal Homeostasis

doi: 10.1074/jbc.m309338200

Figure Lengend Snippet: FIG. 6. Functional characterization of AtOPT3. A, hybridization of an 600-bp AtOPT3 cDNA probe, corresponding to the 3-terminal exon, to RNA extracted from Arabidopsis roots of plants grown in control conditions (Ctl), copper (-Cu), manganese (-Mn), zinc (-Zn), and iron (-Fe) deficiency. The ethidium bromide-stained RNA gel is shown for quantification. B, growth of ctr1 expressing AtOPT3 on YPG-Ura plates supplemented with 10 M CuSO4 compared with the mutant transformed with the vector alone. C, growth of smf1 expressing AtOPT3 on manganese-limited medium, with and without 1 mM EGTA compared with the growth of the mutant transformed with the vector alone.

Article Snippet: Genome-wide Analysis Provides Insight into Metal Transport—We have used Affymetrix Arabidopsis DNA chips containing 8,300 genes (which cover about one-third of the ge- FIG. 7.

Techniques: Functional Assay, Hybridization, Control, Staining, Expressing, Mutagenesis, Transformation Assay, Plasmid Preparation

Journal: bioRxiv

Article Title: Defective BRCA1-mediated DNA end resection drives tandem duplication formation and FANCM synthetic lethality

doi: 10.64898/2026.02.20.706968

Figure Lengend Snippet: A. Schematic of RPA accumulation on ssDNA in response to DNA end resection of a Cas9 induced DSB, targeted to the I-SceI site within the HR reporter at Rosa26 . Intensity of RPA ChIP-seq signal reflects the efficiency of DNA end resection. B. Cas9 DSB-induced RPA ChIP-seq signals in two independent experiments (REP#1 and REP#2) using Brca1 genotypes shown. Brca1 allele abbreviations as in . See STAR Methods for details. C . Quantitation of RPA enrichment averaged over 30 kb window flanking I-SceI site. Signals were normalized to WT for each of the replicates. Statistical analysis: unpaired t -test with Welch’s correction. ns: not significant, p *<0.05.

Article Snippet: The ChIP libraries were prepared according to the NEB Ultra II DNA Library Prep Kit (NEB, #E7645).

Techniques: ChIP-sequencing, Quantitation Assay